Reagents

• 10µg/ml DAPI (4'-6-diamidino-2-phenylindole dihydrochloride) in PBS with 0.6% NP40 (Nonidet P40)

Staining

1. Centrifuge 1 x 10⁶ cells to a pellet and carefully remove supernatant.
2. Loosen pellet.
3. Resuspend pellet in 1ml of DAPI staining solution.
4. Keep on ice until run on flow cytometer.