Reagents

• 1mg/ml MC540 (merocyaninine 540, Sigma Chemical cat# M6390) in H₂O
• 5µl PI (propidium iodide, Sigma Chemical cat# P-4170 in PBS.

Staining

1. Resuspend 1 x 10⁶ cells in 0.375ml culture media.
2. Add 125µl of PI stock (5µg/ml PI in PBS)
3. Add 2.5µl of MC540 stock (1mg/ml MC540 in H₂O
4. Mix.
5. Wait 1 minute.
6. Run on flow cytometer (run MC540 in PE channel).