• The investigator is responsible for having the correct cell density (1 x 106 per ml) and volume (minimum of 300 ul) per tube for analysis using one of the benchtop flow cytometers. Densities and volumes less than the recommended ones cause difficulty in setting up the instrument. Cell density for sorting should be between 5 x 106 and 20 x 106 per ml. See Sample Preparation for Analysis and Sorting for more information.
     
  • The investigator is responsible for bringing the correct compensation tubes for running multi-color experiments. See Compensation Controls for Flow Cytometry for more information.
     
  • The person accompanying the tubes should have intimate knowledge of the samples (cell type, fluorochromes, biohazard status) and goal of the analysis.
     
  • The person accompanying the sample tubes for cell sorting is responsible for making sure that the data look correct on the instrument display screen once the operator has made the final adjustments to amplifier gains and compensation settings. If the instrument needs adjustment, the operator should be informed so that changes can be made to bring the settings into compliance with the investigator's parameters.