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Miltenyi autoMACS
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- Becton Dickinson Accuri C6 with 488nm and 640nm lasers
- Becton Dickinson FACS Aria Fusion
- Becton Dickinson LSR II (VA Satellite Lab) with 405nm, 488nm, and 633nm lasers
- Becton Dickinson LSR II with 488nm, and 633nm lasers
- Becton Dickinson LSR II with 405nm, 488nm, 561nm, and 639nm lasers
- BioRad BioPlex
- Cytek Amnis ImageStreamX MkII
- Cytek Aurora CS
- Cytek Aurora with 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm lasers
- Miltenyi autoMACS
Instrument Details
Magnetic based cell separator
- Useful for pre-enrichment before fluorescence-based cell sorting
Capacity
- 4 x 109 total cells
- 1 x 107 per 100 µl cell concentration
- 2 x 108 total labeled cells
- 1 x 105 minimum number of cells
- 500 µl minimum sample volume
- For positive selection, instrument puts separated cells in 2 ml
- For negative selection, instrument puts separated cells in 2 ml plus the original volume
Sort Programs
- Positive or negative selection for all combinations of high and low antigen expression for recovery or purity