Main navigation
- Reserve this Instrument
- Five-laser Cytek Aurora User Guide
- UI Training Slides
- Five Laser Cytek Aurora Fluorochrome Selection
- Cytek fluorescence spectral viewer (specific to Cytek spectral analyzers)
- Aurora Quick Reference Guide
- Producing tSNE plots in FlowJo v10
- Setting FlowJo 10 Scale Values for Cytek Aurora Files
Instrument Details
Five Excitation Sources
- 20 mW at 355 nm (UV)
- 100 mW at 405 nm (violet)
- 50 mW at 488 nm (blue)
- 50 mW at 561 nm (yellow-green)
- 80 mW at 640 nm (red)
Youtube Tutorials
- SpectroFlo Software Overview
- Create and Unmix an Experiment in SpectroFlo
- Adding Fluorescent Tags
- Group Specific Unstained Controls
- Creating and Using Stored Controls
- Lot and Label Specific Unmixing
- Live Unmixing versus Unmix, Save and Open
- Adjusting Compensation
- Adjusting Gains (when reference controls are offscale)
- Create Experimental Templates
- Extract Autofluorescence
- Export 18 and 22-bit FCS Files
- Volumetric Counting
- Import and Export Exeriments
- Data Management
- Restoring Default User Settings and Worksheets
- Sharing User Settings and Worksheets
- Worksheet Tools
- Batch Analysis
- Merge FCS Files
- Creating and Using Keywords
- Cytometer Menu
- Extra Tools Menu
- Preferences Menu
- Reference Control QC Tools
- Changing CytekAssaySetting
- Create an FCS File from Gated Data
- ReUsing Reference Controls
- Editing Plot Properties
- Changing Bead Lots
- User Management
- New Preferences in SpectroFlo v2.2
- Help Module
- Bubble and Clog Detection
- How to Calibrate Sample Line Depth (CalSIT) for Different Plate Types
64 Fluorescence Emission Detection Channels (providing measurement of the full visible spectrum for each fluorochrome)
- Ultra-violet detector module: 16 channels
- Violet detector module: 16 channels
- Blue detector module: 14 channels
- Yellow-Green detector module: 10 channels
- Red detector module: 8 channels
Automatic Micro-Sampling System (AMS)
- Sample cells from a U-bottom, V-bottom, or flat bottom standard-height 96-well plate
Pulse Processing
- Pulse area and height measurement available for all channels
- Pulse width of forward and side scatter
- Pulse width of one fluorescence parameter for each laser
Microparticle Detection
- Violet side scatter detector for thresholding on microparticles
Volumetric Measurement
- Direct volumetric measurement for cell counting