Video

Web-based flow cytometry training
Animated tutorials presented by BioRad covering the following flow cytometry subjects. (Account creation necessary)

• Overview
• Fluidics
• Optics
• Electronics
• Optical Measurements
• Data Analysis
• Sorting

Introduction to Flow Cytometry (12 minutes)
Animated tutorial presented by Invitrogen (requires a browser that supports Flash)

Data Analysis for Flow Cytometry  (17 minutes)
Animated tutorial presented by Invitrogen (requires a browser that supports Flash)

Multi-color Flow Cytometry  (56 minutes)
Covers all aspects of multi-color experiments from fluorochrome chemistry to controls, divided into chapters, presented by BioRad

Cytek Full Spectrum Panel Design Series (Spectrolearn)

Print Media

• Flow Cytometry Instrumentation – An Overview (published Nov. 2018)
• Flow Cytometry: Principles, Best Practices, and Considerations for Experimental Design (Bethyl)
• Bio-Techne Flow Cytometry Handbook
• Introduction to Flow Cytometry (BioRad)
• Flow Cytometry - A Basic Introduction by Michael G. Ormerod (wiki version)
•    Online version of Howard Shapiro's book "Practical Flow Cytometry"

FlowJo v10

•  FlowJo v10 seminar slides (March 1, 2017) – Graciously provided by Dr. Tim Crawford
• Web based FlowJo tutorials
• Producing tSNE plots in FlowJo v10
• Setting FlowJo 10 Scale Values for Cytek Aurora Files
• FlowJo Tutorials with practice data
• FlowJo University Videos

The Impact of Adjusting PMT Voltages on Spillover and Compensation

The slides at the following link explain why adjusting PMT voltages to lower compensation values does not improve immunofluorescent experiment results.

Impact of PMT Voltages on Spillover and Compensation

Biexponential Scaling

Biexponential scaling is an option that allows log-scaled data that is on or below the baseline to be displayed on scale.

Examples of Biexponential Scaling

Discussion of biexponential scaling option for log-scaled data.

Statistics

Calculation for Comparing Populations (Staining Index)

This calculation can be used for normalizing the relationship between positive and negative populations to compare treated and untreated samples.

SI = (MFI_pos – MFI_neg ) / (2 x SD_neg) 

SI = staining index
MFI = median, geometric-mean, or mean fluorescence intensity
SD = standard deviation.

Note: Population average fluorescence intensity values can be skewed depending on the population distribution. Median fluorescence intensity is less skewed than geometric-mean fluorescence intensity and geometric-mean fluorescence intensity is less skewed than mean fluorescence intensity for log-normal populations (populations that look gaussian when plotted on a log scale).