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Statistics

Calculation for Comparing Populations (Staining Index)

This calculation can be used for normalizing the relationship between positive and negative populations to compare treated and untreated samples.

SI = (MFI_pos – MFI_neg ) / (2 x SD_neg) 

SI = staining index
MFI = median, geometric-mean, or mean fluorescence intensity
SD = standard deviation.

Note: Population average fluorescence intensity values can be skewed depending on the population distribution. Median fluorescence intensity is less skewed than geometric-mean fluorescence intensity and geometric-mean fluorescence intensity is less skewed than mean fluorescence intensity for log-normal populations (populations that look gaussian when plotted on a log scale).

FlowJo v10

• FlowJo v10 seminar slides (March 1, 2017) – Graciously provided by Dr. Tim Crawford
• Web based FlowJo tutorials
• Producing tSNE plots in FlowJo v10
• Setting FlowJo 10 Scale Values for Cytek Aurora Files
• FlowJo Recorded Webinars

Fluorescence Spectral Viewers

• Becton Dickinson
• Thermo Fisher   /   eBiosciences   /  Invitrogen   /   Molecular Probes
• BioLegend
• Evrogen (for fluorescent proteins)
• Chroma
• Semrock
• Cytek (specific to Cytek spectral analyzers)

Fluorochrome Brightness Comparisons

• BioLegend
• BD Biosciences
• Miltenyi

Antibody/Fluorochrome Conjugation Kits

• Expedeon
• Biotium

Antibody Search Engines for Difficult to Find Antibodies

• BenchSi web-based search engine for published antibodies
• Flurosearch

Dye and Probe References

• Molecular Probes Handbook —A Guide to Fluorescent Probes and Labeling Technologies (latest edition)

Mobile Apps for Flow Cytometry and the Lab

• ThermoFisher Scientific apps for flow cytometry and other lab applications