Gene expression in single cells (mouse or human) using eBioscience PrimeFlow^TM

• eBioscience PrimeFlow RNA assay
  • See gene expression heterogeneity at the single-cell level
  • Compare RNA and protein kinetics in the same cell
  • Detect non-coding RNA in cellular subsets
  • Evaluate viral RNA in infected cells
  • Analyze mRNA expression levels when antibody selection is limited
• eBioscience PrimeFlow RNA Assay Technology Validation Paper
• eBioscience PrimeFlow user manual and staining protocol

BD FACSDiscover™ S8 Cell Sorter with BD CellView™ Image Technology

• Becton Dickenson S8 Cell Sorter
  • Image Analysis Capable of Cell Sorting
  • Visualize and sort internalization and cell trafficking
  • Cell-to-cell interactions
  • Spatial and morphological insights
  • Six imaging parameters on the 488nm laser

Flow cytometry combined with image analysis

Amnis produces instruments that combine flow cytometry and image analysis into one unit. This is an instrument the Facility is considering for future services.

• Amnis ImageStream Mark II
  • Combines the speed, statistical power, and fluorescence sensitivity of flow cytometry with the functional insights of high resolution microscopy
  • Can analyze up to 5,000 cells/sec
  • User interface provides real-time plotting and graphical gating (flow cytometry histograms and dot plots), as well as images of every cell
  • Works with sample volumes of 20-200 ul
  • 60x/40x/20x magnification options
  • Accepts up to 7 lasers
• ImageStream Applications

Miltenyi Biotec MACSQuant® Tyto® Cell Sorter

Gentler chip based cell sorter

• MACSQuant
  • Microchips allow for low pressure, gentle sorts
  • Sort cartridge are single use and sterile
  • 405nm, 488nm, 638nm lasers for two scatter and 10 fluorescent parameters