Optimised Multicolor Immunofluorescence Panels

• The goal of a published OMIP:
  • To alleviate the development time for researchers who wish to use the same (or highly similar) panels
  • To provide a starting point for the creation of novel OMIPs
  • To provide a mechanism for attribution to the developers of the panel via citation of the publication
• Publication of Optimized Multicolor Immunofluorescence Panels
• OMIPs - Orchestrating Multiplexity In Polychromatic science

Listing of all Optimised Multicolor Immunofluorescence Panels

• OMIP-001: Quality and Phenotype of Ag-Responsive Human T-Cells
• OMIP-002: Phenotypic Analysis of Specific Human CD8+ T-Cells Using Peptide-MHC Class I Multimers for Any of Four Epitopes
• OMIP-003: Phenotypic Analysis of Human Memory B Cells
• OMIP-004: In-Depth Characterization of Human T Regulatory Cells
• OMIP-005: Quality and Phenotype of Antigen-Responsive Rhesus Macaque T Cells
• OMIP-006: Phenotypic Subset Analysis of Human T Regulatory Cells via Polychromatic Flow Cytometry
• OMIP-007: Phenotypic Analysis of Human Natural Killer Cells
• OMIP-008: Measurement of Th1 and Th2 Cytokine Polyfunctionality of Human T Cells
• OMIP-009: Characterization of Antigen-Specific Human T-Cells
• OMIP-010: A New 10-Color Monoclonal Antibody Panel for Polychromatic Immunophenotyping of Small Hematopoietic Cell Samples
• OMIP-011: Characterization of Circulating Endothelial Cells (CECs) in Peripheral Blood
• OMIP-012: Phenotypic and Numeric Determination of Human Leukocyte Reconstitution in Humanized Mice
• OMIP-013: Differentiation of Human T-Cells
• OMIP-014: Validated Multifunctional Characterization of Antigen-Specific Human T Cells by Intracellular Cytokine Staining
• OMIP-015: Human Regulatory and Activated T-Cells Without Intracellular Staining
• OMIP-016: Characterization of Antigen-Responsive Macaque and Human T-cells
• OMIP-017: Human CD41 Helper T-cell Subsets Including Follicular Helper Cells
• OMIP-018: Chemokine Receptor Expression on Human T Helper Cells
• OMIP-019: Quantification of Human cdT-Cells, iNKT-Cells, and Hematopoietic Precursors
• OMIP-020: Phenotypic Characterization of Human cd T-cells by Multicolor Flow Cytometry
• OMIP-021: Simultaneous Quantification of Human Conventional and Innate-Like T-Cell Subsets
• OMIP-022: Comprehensive Assessment of Antigen-Specific Human T-Cell Functionality and Memory
• OMIP-023: 10-Color, 13 Antibody Panel for In-depth Phenotyping of Human Peripheral Blood Leukocytes
• OMIP-024: Pan-Leukocyte Immunophenotypic Characterization of PBMC Subsets in Human Samples
• OMIP-025: Evaluation of Human T- and NK-Cell Responses Including Memory and Follicular Helper Phenotype by Intracellular Cytokine Staining
• OMIP-026: Phenotypic Analysis of B and Plasma Cells in Rhesus Macaques
• OMIP-027: Functional analysis of human natural killer cells
• OMIP-028: Activation Panel for Rhesus Macaque NK Cell Subsets
• OMIP-029: Human NK-Cell Phenotypization
• OMIP-030: Characterization of Human T Cell Subsets via Surface Markers
• OMIP-031: Immunologic Checkpoint Expression on Murine Effector and Memory T-Cell Subsets
• OMIP-032: Two Multi-Color Immunophenotyping Panels for Assessing the Innate and Adaptive Immune Cells in the Mouse Mammary Gland
• OMIP-033: A Comprehensive Single Step Staining Protocol for Human T- and B-Cell Subsets
• OMIP-034: Comprehensive Immune Phenotyping of Human Peripheral Leukocytes by Mass Cytometry for Monitoring Immunomodulatory Therapies
• OMIP-035: Functional Analysis of Natural Killer Cell Subsets in Macaques Immunomodulatory Therapies
• OMIP-036: Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets
• OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets
• OMIP-038: Innate Immune Assessment with a 14 Color Flow Cytometry Panel
• OMIP-039: Detection and Analysis of Human Adaptive NKG2C+ Natural Killer Cells
• OMIP-040: Optimized Gating of Human Prostate Cellular Subpopulations
• OMIP-041: Optimized multicolor immunofluorescence panel rat microglial staining protocol
• OMIP-042: 21‐color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood
• OMIP-043: Identification of human antibody secreting cell subsets
• OMIP-044: 28‐color immunophenotyping of the human dendritic cell compartment
• OMIP-045: Characterizing human head and neck tumors and cancer cell lines with mass cytometry
• OMIP-046: Characterization of invariant T cell subset activation in humans
• OMIP-047: High‐Dimensional phenotypic characterization of B cells
• OMIP-048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry
• OMIP-049: Analysis of Human Myelopoiesis and Myeloid Neoplasms
• OMIP-050: A 28‐color/30‐parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules
• OMIP-051: 28-Color Flow Cytometry Panel to Characterize B Cells and Myeloid Cells
• OMIP-052: An 18-Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques
• OMIP-053: Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg Subsets
• OMIP-054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry
• OMIP-055: Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood.
• OMIP-056: Evaluation of Human Conventional T Cells, Donor‐Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining
• OMIP-057: Mouse γδ T‐Cell Development Characterized by a 14 Color Flow Cytometry Panel
• OMIP‐058: 30‐Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells
• OMIP‐059: Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining Panel
• OMIP-060: 30-Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T Cells.
• OMIP‐061: 20‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting Cells
• OMIP‐062: A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples
• OMIP‐063: 28‐Color Flow Cytometry Panel for Broad Human Immunophenotyping
• OMIP‐064: A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells
• OMIP‐065: Dog Immunophenotyping and T‐Cell Activity Evaluation with a 14‐Color Panel
• OMIP‐066: Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood
• OMIP‐067: 28‐Color Flow Cytometry Panel to Evaluate Human T‐Cell Phenotype and Function
• OMIP‐068: High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic Infection
• OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood
• OMIP‐070: NKp46‐Based 27‐Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues
• OMIP 071: A 31-Parameter Flow Cytometry Panel for In-Depth Immunophenotyping of Human T-Cell Subsets Using Surface Markers
• OMIP 072: A 15-color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemia
• OMIP 073: Analysis of human thymocyte development with a 14-color flow cytometry panel
• OMIP 074: Phenotypic analysis of IgG and IgA subclasses on human B cells
• OMIP 075: A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primates
• OMIP 076: High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsets
• OMIP 077: Definition of all principal human leukocyte populations using a broadly applicable 14-color panel
• OMIP 078: A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells
• OMIP-079: Cell cycle of CD4+ and CD8+ naïve/memory T cell subsets, and of Treg cells from mouse spleen
• OMIP-080: 29-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantation
• OMIP - 081: A new 21-monoclonal antibody 10-color panel for diagnostic polychromatic immunophenotyping
• OMIP 082: A 25-color phenotyping to define human innate lymphoid cells, natural killer cells, mucosal-associated invariant T cells, and γδ T cells from freshly isolated human intestinal tissue
• OMIP 083: A 21-marker 18-color flow cytometry panel for in-depth phenotyping of human peripheral monocytes
• OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells
• OMIP-085: Cattle B-cell phenotyping by an 8-color panel
• OMIP-086: Full spectrum flow cytometry for high-dimensional immunophenotyping of mouse innate lymphoid cells
• OMIP-087: Thirty-two parameter mass cytometry panel to assess human CD4 and CD8 T cell activation, memory subsets, and helper subsets
• OMIP-088: Twenty-target imaging mass cytometry panel for major cell populations in mouse formalin fixed paraffin embedded liver
• OMIP-089: Cattle T-cell phenotyping by an 8-color panel
• OMIP-090: A 20-parameter flow cytometry panel for rapid analysis of cell diversity and homing capacity in human conventional and regulatory T cells
• OMIP-91: A 27-color flow cytometry panel to evaluate the phenotype and function of human conventional and unconventional T-cells
• OMIP-92: Characterization of rat macrophage subsets, lymphocytes, and granulocytes in bronchoalveolar lavage fluid
• OMIP-93: A 41-color high parameter panel to characterize various co-inhibitory molecules and their ligands in the lymphoid and myeloid compartment in mice
• OMIP-94: Twenty-four-color (thirty-marker) panel for deep immunophenotyping of immune cells in human peripheral blood
• OMIP-095: 40-Color spectral flow cytometry delineates all major leukocyte populations in murine lymphoid tissues
• OMIP-096: A 24-color flow cytometry panel to identify and characterize CD4+ and CD8+ tissue-resident T cells in human skin, intestinal, and type II mucosal tissue
• OMIP-097: High-parameter phenotyping of human platelets by spectral flow cytometry
• OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping
• OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cells
• OMIP-100: A flow cytometry panel to investigate human neutrophil subsets
• OMIP-101: 27-color flow cytometry panel for immunophenotyping of major leukocyte populations in fixed whole blood
• OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells